Avoidance of sulfur loss during ammonia treatment of oligonucleotide phosphorothioates.
نویسندگان
چکیده
Sulfur loss during the unblocking of phosphorothioate analogues of oligonucleotides with concentrated aqueous ammonia can be completely suppressed by the addition of 2-mercaptoethanol.
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عنوان ژورنال:
- Nucleic acids research
دوره 25 14 شماره
صفحات -
تاریخ انتشار 1997